Day 0 | Assembly of the device Air bubbles removal through manual filling with 70% ethanol and isopropanol Injection of pluronic solution (10 mg/mL) Incubation for 24 h at room temperature |
Day 1 | Flush with water to remove excess pluronic Fill with cell medium (DMEM/F12) RT treatment of 0 to 8 Gy (8 Gy considered default) |
Day 1–X | Incubation at 37 °C for 0 to 6 days (6 days considered default) |